Highlighted Publications

Multimodal Spatial Profiling Reveals Immune Suppression and Microenvironment Remodeling in Fallopian Tube Precursors to High-Grade Serous Ovarian Carcinoma

Kader T, Lin JR, Hug CB, Coy S, Chen YA, de Bruijn I, Shih N, Jung E, Pelletier RJ, Lopez Leon M, Mingo G, Omran DK, Lee JS, Yapp C, Satravada BA, Kundra R, Xu Y, Chan S, Tefft JB, Muhlich JL, Kim SH, Gysler SM, Agudo J, Heath JR, Schultz N, Drescher CW, Sorger PK, Drapkin R, Santagata S.
Cancer Discovery. 2024 Dec 20. PMID: 39704522.

Epigenetic and Oncogenic Inhibitors Cooperatively Drive Differentiation and Kill KRAS-Mutant Colorectal Cancers

Loi P, Schade AE, Rodriguez CL, Krishnan A, Perurena N, Nguyen VTM, Xu Y, Watanabe M, Davis RA, Gardner A, Pilla NF, Mattioli K, Popow O, Gunduz N, Lannagan TRM, Fitzgerald S, Sicinska ET, Lin JR, Tan W, Brais LK, Haigis KM, Giannakis M, Ng K, Santagata S, Helin K, Sansom OJ, Cichowski K.
Cancer Discov. 2024 Dec 2. PMCID: PMC11609823

Quality control for single-cell analysis of high-plex tissue profiles using CyLinter

Baker GJ, Novikov E, Zhao Z, Vallius T, Davis JA, Lin JR, Muhlich JL, Mittendorf EA, Santagata S, Guerriero JL, Sorger PK.
Nat Methods. 2024 Oct 30; PMID: 39478175

Ovarian cancer-derived IL-4 promotes immunotherapy resistance

Mollaoglu G, Tepper A, Falcomatà C, Potak HT, Pia L, Amabile A, Mateus-Tique J, Rabinovich N, Park MD, LaMarche NM, Brody R, Browning L, Lin JR, Zamarin D, Sorger PK, Santagata S, Merad M, Baccarini A, Brown BD.
Cell. 2024 Oct 25. PMID: 39481380

State-Dependent Inhibition of Nav1.8 Sodium Channels by VX-150 and VX-548

Vaelli P, Fujita A, Jo S, Zhang HXB, Osorno T, Ma X, Bean BP.
Mol Pharmacol. 2024 Nov 18. PMCID: PMC11585256

AKT and EZH2 inhibitors kill TNBCs by hijacking mechanisms of involution

Schade AE, Perurena N, Yang Y, Rodriguez CL, Krishnan A, Gardner A, Loi P, Xu Y, Nguyen VTM, Mastellone GM, Pilla NF, Watanabe M, Ota K, Davis RA, Mattioli K, Xiang D, Zoeller JJ, Lin JR, Morganti S, Garrido-Castro AC, Tolaney SM, Li Z, Barbie DA, Sorger PK, Helin K, Santagata S, Knott SRV, Cichowski K.
Nature. 2024 Oct 9; PMID: 39385030

A CRISPRi/a screening platform to study cellular nutrient transport in diverse microenvironments

Chidley C, Darnell AM, Gaudio BL, Lien EC, Barbeau AM, Vander Heiden MG, Sorger PK.
Nat Cell Biol. 2024 Apr 11; PMID: 38605144

Nociceptor-immune interactomes reveal insult-specific immune signatures of pain

Jain A, Gyori BM, Hakim S, Jain A, Sun L, Petrova V, Bhuiyan SA, Zhen S, Wang Q, Kawaguchi R, Bunga S, Taub DG, Ruiz-Cantero MC, Tong-Li C, Andrews N, Kotoda M, Renthal W, Sorger PK, Woolf CJ.
Nat Immunol. 2024 May 28. PMCID: PMC11224023

Phase II Study of Eribulin plus Pembrolizumab in Metastatic Soft Tissue Sarcomas: Clinical Outcomes and Biological Correlates

Haddox CL, Nathenson MJ, Mazzola E, Lin JR, Baginska J, Nau A, Weirather JL, Choy E, Marino-Enriquez A, Morgan JA, Cote GM, Merriam P, Wagner AJ, Sorger PK, Santagata S, George S.
Clin Cancer Res. 2024 Jan 18. PMCID: PMC10982640

Targeting TBK1 to overcome resistance to cancer immunotherapy

Sun Y, Revach OY, Anderson S, Kessler EA, Wolfe CH, Jenney A, Mills CE, Robitschek EJ, Davis TGR, Kim S, Fu A, Ma X, Gwee J, Tiwari P, Du PP, Sindurakar P, Tian J, Mehta A, Schneider AM, Yizhak K, Sade-Feldman M, LaSalle T, Sharova T, Xie H, Liu S, Michaud WA, Saad-Beretta R, Yates KB, Iracheta-Vellve A, Spetz JKE, Qin X, Sarosiek KA, Zhang G, Kim JW, Su MY, Cicerchia AM, Rasmussen MQ, Klempner SJ, Juric D, Pai SI, Miller DM, Giobbie-Hurder A, Chen JH, Pelka K, Frederick DT, Stinson S, Ivanova E, Aref AR, Paweletz CP, Barbie DA, Sen DR, Fisher DE, Corcoran RB, Hacohen N, Sorger PK, Flaherty KT, Boland GM, Manguso RT, Jenkins RW.
Nature. 2023 Mar. PMCID: PMC10171827

Changing fitness effects of mutations through long-term bacterial evolution

Couce A, Limdi A, Magnan M, Owen SV, Herren CM, Lenski RE, Tenaillon O, Baym M
Science. 2024 Jan 26. PMID: 38271521

Development and validation of time-to-event models to predict metastatic recurrence of localized cutaneous melanoma

Wan G, Leung BW, DeSimone MS, Nguyen N, Rajeh A, Collier MR, Rashdan H, Roster K, Zhou X, Moseley CB, Nirmal AJ, Pelletier RJ, Maliga Z, Marko-Varga G, Németh IB, Tsao H, Asgari MM, Gusev A, Stagner AM, Lian CG, Hurlbert MS, Liu F, Yu KH, Sorger PK, Semenov YR.
J Am Acad Dermatol. 2023 Oct 3;S0190-9622(23)02881–5. PMID: 37797836

High-plex immunofluorescence imaging and traditional histology of the same tissue section for discovering image-based biomarkers

Lin JR, Chen YA, Campton D, Cooper J, Coy S, Yapp C, Tefft JB, McCarty E, Ligon KL, Rodig SJ, Reese S, George T, Santagata S, Sorger PK
Nature Cancer. 2023 Jul. PMID: 37349501

OpenFold: retraining AlphaFold2 yields new insights into its learning mechanisms and capacity for generalization

Ahdritz G, Bouatta N, Floristean C, Kadyan S, Xia Q, Gerecke W, O’Donnell TJ, Berenberg D, Fisk I, Zanichelli N, Zhang B, Nowaczynski A, Wang B, Stepniewska-Dziubinska MM, Zhang S, Ojewole A, Guney ME, Biderman S, Watkins AM, Ra S, Lorenzo PR, Nivon L, Weitzner B, Ban YEA, Chen S, Zhang M, Li C, Song SL, He Y, Sorger PK, Mostaque E, Zhang Z, Bonneau R, AlQuraishi M.
Nat Methods. 2024 May 14. PMID: 38744917

Multi-range ERK responses shape the proliferative trajectory of single cells following oncogene induction.

Chen JY, Hug C, Reyes J, Tian C, Gerosa L, Fröhlich F, Ponsioen B, Snippert HJG, Spencer SL, Jambhekar A, Sorger PK, Lahav G.
Cell Rep. 2023 Mar 28;42(3):112252. PMCID: PMC10153468

Multiplexed 3D atlas of state transitions and immune interaction in colorectal cancer

Lin JR, Wang S, Coy S, Chen YA, Yapp C, Tyler M, Nariya MK, Heiser CN, Lau KS, Santagata S, Sorger PK.
Cell. 2023 Jan 19. PMID: 36669472

Sequential apoptotic and multiplexed proteomic evaluation of single cancer cells

Lecky E, Mukherji A, German R, Antonellis G, Lin JR, Yorsz M, McQueeney KE, Ryan J, Ng K, Sicinska E, Sorger PK, Letai A, Bhola PD.
Science Advances. 2023 Jun 23;9(25): eadg4128. PMID: 37352344

Proteomic profiling across breast cancer cell lines and models

Kalocsay M, Berberich MJ, Everley RA, Nariya MK, Chung M, Gaudio B, Victor C, Bradshaw GA, Eisert RJ, Hafner M, Sorger PK, Mills CE, Subramanian K.
Sci Data. 2023 Aug 4;10(1):514. PMCID: PMC10403526

Lymphocyte networks are dynamic cellular communities in the immunoregulatory landscape of lung adenocarcinoma

Gaglia G, Burger ML, Ritch CC, Rammos D, Dai Y, Crossland GE, Tavana SZ, Warchol S, Jaeger AM, Naranjo S, Coy S, Nirmal AJ, Krueger R, Lin JR, Pfister H, Sorger PK, Jacks T, Santagata S.
Cancer Cell. 2023 Apr 8. PMID: 37059105

A hybrid structure-based machine learning approach for predicting kinase inhibition by small molecules

Liu C, Kutchukian P, Nguyen ND, AlQuraishi M, Sorger PK
J Chem Inf Model. 2023 Sep 11;63(17):5457–5472. PMCID: PMC10498990

Automated assembly of molecular mechanisms at scale from text mining and curated databases

Bachman JA, Gyori BM, Sorger PK.
Mol Syst Biol. 2023 May 9. PMCID: PMC10167483.

Evolution of delayed resistance to immunotherapy in a melanoma responder

Liu D, Lin JR, Robitschek EJ, Kasumova GG, Heyde A, Shi A, Kraya A, Zhang G, Moll T, Frederick DT, Chen YA, Wang S, Schapiro D, Ho LL, Bi K, Sahu A, Mei S, Miao B, Sharova T, Alvarez-Breckenridge C, Stocking JH, Kim T, Fadden R, Lawrence D, Hoang MP, Cahill DP, Malehmir M, Nowak MA, Brastianos PK, Lian CG, Ruppin E, Izar B, Herlyn M, Van Allen EM, Nathanson K, Flaherty KT, Sullivan RJ, Kellis M, Sorger PK, Boland GM
Nat Med. 2021 Jun. PMCID: PMC8474080

Mechanistic model of MAPK signaling reveals how allostery and rewiring contribute to drug resistance

Fröhlich F, Gerosa L, Muhlich J, Sorger PK.
Molecular Systems Biology. 2023 Jan 26;e10988. PMID: 36700386

MITI minimum information guidelines for highly multiplexed tissue images

Schapiro D, Yapp C, Sokolov A, Reynolds SM, Chen YA, Sudar D, Xie Y, Muhlich J, Arias-Camison R, Arena S, Taylor AJ, Nikolov M, Tyler M, Lin JR, Burlingame EA, Human Tumor Atlas Network, Chang YH, Farhi SL, Thorsson V, Venkatamohan N, Drewes JL, Pe’er D, Gutman DA, Herrmann MD, Gehlenborg N, Bankhead P, Roland JT, Herndon JM, Snyder MP, Angelo M, Nolan G, Swedlow JR, Schultz N, Merrick DT, Mazzili SA, Cerami E, Rodig SJ, Santagata S, Sorger PK.
Nat Methods. 2022 Mar. PMCID: PMC9009186

Causal inference in medical records and complementary systems pharmacology for metformin drug repurposing towards dementia

Charpignon ML, Vakulenko-Lagun B, Zheng B, Magdamo C, Su B, Evans K, Rodriguez S, Sokolov A, Boswell S, Sheu YH, Somai M, Middleton L, Hyman BT, Betensky RA, Finkelstein SN, Welsch RE, Tzoulaki I, Blacker D, Das S, Albers MW.
Nature Communications. 2022 Dec 10. PMCID: PMC9741618

Single cell spatial analysis reveals the topology of immunomodulatory purinergic signaling in glioblastoma

Coy S, Wang S, Stopka SA, Lin JR, Yapp C, Ritch CC, Salhi L, Baker GJ, Rashid R, Baquer G, Regan M, Khadka P, Cole KA, Hwang J, Wen PY, Bandopadhayay P, Santi M, De Raedt T, Ligon KL, Agar NYR, Sorger PK, Touat M, Santagata S.
Nat Commun. 2022 Aug 16. PMCID: PMC9381513

Receptor-driven ERK pulses reconfigure MAPK signaling and enable persistence of drug-adapted BRAF-mutant melanoma cells

Gerosa L, Chidley C, Fröhlich F, Sanchez G, Lim SK, Muhlich J, Chen JY, Vallabhaneni S, Baker GJ, Schapiro D, Atanasova MI, Chylek LA, Shi T, Yi L, Nicora CD, Claas A, Ng TSC, Kohler RH, Lauffenburger DA, Weissleder R, Miller MA, Qian WJ, Wiley HS, Sorger PK.
Cell Syst. 2020 Nov 18. PMCID: PMC8009031

UnMICST: Deep learning with real augmentation for robust segmentation of highly multiplexed images of human tissues.

Yapp C, Novikov E, Jang W-D, Vallius T, Chen Y-A, Cicconet M, Maliga Z, Jacobson CA, Wei D, Santagata S, Pfister H, Sorger PK.
Communications Biology. 2022 Nov 18;5(1):1263. PMID: 36400937. PMCID: PMC9674686.

Lineage abundance estimation for SARS-CoV-2 in wastewater using transcriptome quantification techniques

Baaijens JA, Zulli A, Ott IM, Nika I, van der Lugt MJ, Petrone ME, Alpert T, Fauver JR, Kalinich CC, Vogels CBF, Breban MI, Duvallet C, McElroy KA, Ghaeli N, Imakaev M, Mckenzie-Bennett MF, Robison K, Plocik A, Schilling R, Pierson M, Littlefield R, Spencer ML, Simen BB, Yale SARS-CoV-2 Genomic Surveillance Initiative, Hanage WP, Grubaugh ND, Peccia J, Baym M.
Genome Biology. 2022 Nov 8;23(1):236. PMCID: PMC9643916

Single-sequence protein structure prediction using a language model and deep learning

Chowdhury R, Bouatta N, Biswas S, Floristean C, Kharkar A, Roy K, Rochereau C, Ahdritz G, Zhang J, Church GM, Sorger PK, AlQuraishi M.
Nature Biotechnology. 2022 Nov. PMID: 36192636

Multiplexed and reproducible high content screening of live and fixed cells using Dye Drop

Mills CE, Subramanian K, Hafner M, Niepel M, Gerosa L, Chung M, Victor C, Gaudio B, Yapp C, Nirmal AJ, Clark N, Sorger PK.
Nature Communications. 2022 Nov 14. PMCID: PMC9663587

Fides: Reliable trust-region optimization for parameter estimation of ordinary differential equation models

Fröhlich, F. & Sorger, PK.
PLoS Computational Biology. 2022 Jul 13;18(7):e1010322. PMID: 35830470. PMCID: PMC9312381

Narrative online guides for the interpretation of digital-pathology images and tissue-atlas data.

Rashid R, Chen Y-A, Hoffer J, Muhlich JL, Lin J-R, Krueger R, Pfister H, Mitchell R, Santagata S, Sorger PK.
Nature Biomedical Engineering. 2022 May. PMCID: 9079188.

The spatial landscape of progression and immunoediting in primary melanoma at single cell resolution.

Nirmal AJ, Maliga Z, Vallius T, Quattrochi B, Chen AC, Jacobson CA, Pelletier RJ, Yapp C, Arias-Camison R, Chen Y-A, Lian CG, Murphy GF, Santagata S, Sorger PK.
Cancer Discovery. 2022 Apr 11. PMCID: PMC9167783.

Response and mechanisms of resistance to larotrectinib and selitrectinib in metastatic undifferentiated sarcoma harboring oncogenic fusion of NTRK1

Hemming ML, Nathenson MJ, Lin JR, Mei S, Du Z, Malik K, Marino-Enriquez A, Jagannathan JP, Sorger PK, Bertagnolli M, Sicinska E, Demetri GD, Santagata S.
JCO Precis Oncol. 2020 Feb 14. PMCID: PMC7055910

Cancer patient survival can be parametrized to improve trial precision and reveal time-dependent therapeutic effects

Plana D, Fell G, Alexander BM, Palmer AC, Sorger PK.
Nature Communications. 2022 Feb 15. PMCID: PMC8847344

Prophages encode phage-defense systems with cognate self-immunity.

Owen S V, Wenner N, Dulberger C, Rodwell E, Bowers-Barnard A, Quinones-Olvera N, Rigden DJ, Rubin, EJ, Garner EC, Baym, M, Hinton JCD.
Cell Host Microbe. 2021 Nov 10;29(11):1620-1633.e8.

Systematic measurement of combination-drug landscapes to predict in vivo treatment outcomes for tuberculosis.

Larkins-Ford J, Greenstein T, Van N, Degefu YN, Olson MC, Sokolov A, Aldridge BB.
Cell Systems. 2021 Aug 21. PMCID: PMC8617591.

Metabolic perturbations sensitize triple-negative breast cancers to apoptosis induced by BH3 mimetics.

Daniels VW, Zoeller JJ, van Gastel N, McQueeney KE, Parvin S, Potter DS, Fell GG, Ferreira VG, Yilma B, Gupta R, Spetz J, Bhola PD, Endress JE, Harris IS, Carrilho E, Sarosiek KA, Scadden DT, Brugge JS, Letai A.
Science Signaling. 2021 Jun 8;14(686):eabc7405. doi: 10.1126/scisignal.abc7405. PMID: 34103421. PMCID: PMC8285073.

Machine learning identifies candidates for drug repurposing in Alzheimer’s disease.

Rodriguez S, Hug C, Todorov P, Moret N, Boswell SA, Evans K, Zhou G, Johnson NT, Hyman BT, Sorger PK, Albers MW, Sokolov A.
Nature Communications. 2021 Feb 15. PMCID: PMC7884393.

Targeting immunosuppressive macrophages overcomes PARP inhibitor resistance in BRCA1-associated triple-negative breast cancer.

Mehta, AK, Cheney, EM, Hartl, CA, Pantelidou, C, Oliwa, M, Castrillon, JA, Lin, JR, Hurst, KE, … Mittendorf EA, Sorger, PK, Shapiro, GI, Guerriero, JL.
Nature Cancer. 2021 Jan;2(1):66-82. doi: 10.1038/s43018-020-00148-7. PMID: 33738458. PMCID: PMC7963404.

A switch in p53 dynamics marks cells that escape from DSB-induced cell cycle arrest.

Tsabar M, Mock CS, Venkatachalam V, Reyes J, Karhohs KW, Oliver TG, Regev A, Jambhekar A, Lahav G.
Cell Reports. 2020 Aug 4;32(5):107995. doi: 10.1016/j.celrep.2020.107995. 32(5):107995. PMID: 32755587; PMCID: PMC7521664.

SYLARAS: A platform for the statistical analysis and visual display of systemic immunoprofiling data and its application to glioblastoma.

Baker GJ, Muhlich JL, Palaniappan SK, Moore JK, Davis SH, Santagata S, Sorger PK.
Cell Systems. 2020 Sep 23;11(3):272-285.e9. doi: 10.1016/j.cels.2020.08.001. PMID: 32898474. PMCID: PMC7565356.