Research

Tissue Profiling & Imaging Methods

LSP researchers are developing high-plex methods that combine spatial imaging and transcriptomics to advance our understanding of the tumor-immune microenvironment.

The LSP is pioneering new methods for highly-multiplexed tissue profiling that capture precise spatial molecular information from 2D and 3D human tissue specimens. LSP researchers invented tissue-based cyclic immunofluorescence (CyCIF), which can stain and image over 60 proteins at single-cell resolution in human tissue. CyCIF has revealed insights into tumor biology, immunology, and the role of inflammation in disease.

We recently built upon this work with 3D-CyCIF, a method that enables a 3-dimensional view of the sub-cellular arrangement of organelles within cells of the tumor microenvironment. 3D CyCIF has revealed novel biology, showing that 3D tissue architecture impacts cell-type identification and neighborhood analysis.

We are also working to integrate spatial biology with clinical workflows. We recently developed the Orion method, which can collect rapidly high-plex immunofluorescence and diagnostic-grade H&E images from the same tissue section. We see Orion as a critical step towards bringing precision diagnostics to the clinic.

To learn more about our methods and view our tissue profiling data, visit the Harvard Tissue Atlas.

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Maliga, Z., Nirmal, A. J., Ericson, N. G., Boswell, S. A., U’Ren, L., Podyminogin, R., Chow, J., Chen, Y.-A., Chen, A. A., Weinstock, D. M., Lian, C. G., Murphy, G. F., Kaldjian, E. P., Santagata, S., & Sorger, P. K. (2021). Micro-region transcriptomics of fixed human tissue using Pick-Seq [Preprint]. bioRxiv. https://doi.org/10.1101/2021.03.18.431004

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Research

Tissue Profiling & Imaging Methods

LSP researchers are developing high-plex methods that combine spatial imaging and transcriptomics to advance our understanding of the tumor-immune microenvironment.

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